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Human TBCEL antibody

CAT: 0112-ATGA0351-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0351-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tubulin-specific chaperone cofactor E-like protein, also known as TBCEL, is a factor that regulates the microtubule cytoskeleton in determining cell behavior.
Product Name Alternative
Tubulin-specific chaperone cofactor E-like protein, El, LRRC35
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human TBCEL (1-424aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT1B10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
TBCEL, ATGA0351-10 µg, ATGA0351-20 µg, ATGA0351-50 µg, ATGA0351-100 µg, ATGA0351-250 µg, ATGA0351-500 µg, ATGA0351-1 mg, ATGA0351-10, ATGA0351-20, ATGA0351-50, ATGA0351-100, ATGA0351-250, ATGA0351-500, ATGA0351-1
References & Citations
Bartolini F., et al. (2005) J Biomed Sci. 118: 1197-1207.; ; Ota T., et al. (2004) Nat Genet. 36: 40-45.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_689928
Uniprot Accession Number
Q5QJ74
WB Description
The Recombinant Human TBCEL (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCEL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human TBCEL; ; Lane 2.: PC3 cell lysate; ; Lane 3.: LNCap cell lysate; ; Lane 4 : 293T cell lysate; ; Lane 5.: Mouse testis lysate; ; Lane 6.: Mouse Kidney lysate
FACS Description
Flow cytometry analysis of TBCEL in PC3 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of TBCEL in PC3 cells. The cell was stained with ATGA0351 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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