Human TBCEL antibody

• Description:

  Tubulin-specific chaperone cofactor E-like protein, also known as TBCEL, is a factor that regulates the microtubule cytoskeleton in determining cell behavior.

• Product Name Alternative:

  Tubulin-specific chaperone cofactor E-like protein, El, LRRC35

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human TBCEL (1-424aa) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  AT1B10

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, ICC/IF, FACS

• Purification Method:

  By protein-A affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  TBCEL, ATGA0351-10 µg, ATGA0351-20 µg, ATGA0351-50 µg, ATGA0351-100 µg, ATGA0351-250 µg, ATGA0351-500 µg, ATGA0351-1 mg, ATGA0351-10, ATGA0351-20, ATGA0351-50, ATGA0351-100, ATGA0351-250, ATGA0351-500, ATGA0351-1

• References & Citations:

  Bartolini F., et al. (2005) J Biomed Sci. 118: 1197-1207.; ; Ota T., et al. (2004) Nat Genet. 36: 40-45.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Cytoskeleton

• NCBI Accession Number:

  NP_689928

• Uniprot Accession Number:

  Q5QJ74

• WB Description:

  The Recombinant Human TBCEL (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCEL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human TBCEL; ; Lane 2.: PC3 cell lysate; ; Lane 3.: LNCap cell lysate; ; Lane 4 : 293T cell lysate; ; Lane 5.: Mouse testis lysate; ; Lane 6.: Mouse Kidney lysate

• FACS Description:

  Flow cytometry analysis of TBCEL in PC3 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

• IF Description:

  ICC/IF analysis of TBCEL in PC3 cells. The cell was stained with ATGA0351 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .