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Human PMVK antibody

CAT: 0112-ATGA0349-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0349-050Size:50 µL
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Description
Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.
Product Name Alternative
Phosphomevalonate kinase, HuMPMKI, PMK, PMKA, PMKASE, Phosphomevalonate kinase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PMVK (1-192aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT21C10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PMVK, ATGA0349-10 µg, ATGA0349-20 µg, ATGA0349-50 µg, ATGA0349-100 µg, ATGA0349-250 µg, ATGA0349-500 µg, ATGA0349-1 mg, ATGA0349-10, ATGA0349-20, ATGA0349-50, ATGA0349-100, ATGA0349-250, ATGA0349-500, ATGA0349-1
References & Citations
Cho YK., et al. (2001) J Biol chem. 278: 4510-4515.; ; Hogenboom S., et al. (2004) J Lipid Res. 45: 697-705.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_006547
Uniprot Accession Number
Q15126
WB Description
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : Jukat cell lysate; ; Lane 4. : MCF7 cell lysate; ; Lane 5. : A549 cell lysate
FACS Description
Flow cytometry analysis of PMVK in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of PMVK in PC3 cells. The cell was stained with ATGA0349 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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