Human EEF1A1 antibody

CAT: 0112-ATGA0347-050Size: 50 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0347-050Size:50 µL
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
Eukaryotic translation elongation factors 1 alpha, eEF1A1 and eEF1A2, are not only translation factors but also pleiotropic proteins that are highly expressed in human tumors, including breast cancer, ovarian cancer, and lung cancer. eEF1A1 modulates cytoskeleton, exhibits chaperone-like activity and also controls cell proliferation and cell death. Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells.
Product Name Alternative
Eukaryotic translation elongation factor 1 alpha 1, Leukocyte receptor cluster (LRC) member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, EEF1A, EF1A, LENG7, EE1A1, EF1A1, EF1alpha1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human EEF1A1 (1-462aa) purified from E.coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT23C11
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EEF1A1, ATGA0347-10 µg, ATGA0347-20 µg, ATGA0347-50 µg, ATGA0347-100 µg, ATGA0347-250 µg, ATGA0347-500 µg, ATGA0347-1 mg, ATGA0347-10, ATGA0347-20, ATGA0347-50, ATGA0347-100, ATGA0347-250, ATGA0347-500, ATGA0347-1
References & Citations
Huang Y., et al. (2012) Zhonqquo Shi Yan Xue Ye Xue Za Zhi. 20 (4) : 835-841.; ; Browne GJ., et al. (2002) Eur J Biochem. 269 (22) : 5360-5368.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001393
Uniprot Accession Number
P68104
WB Description
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EEF1A1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: A549 cell lysate ; ; Lane 3.: Raji cell lysate ; ; Lane 4.: THP-1 cell lysate ; ; Lane 5.: MCF-7 cell lysate
IF Description
ICC/IF analysis of EEF1A1 in A549 cells. The cell was stained with ATGA0347 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

Related Products

CatalogName
ATGP2613-500Recombinant human EF-1 alpha1/EEF1A1 protein
ATGP2613-020Recombinant human EF-1 alpha1/EEF1A1 protein
ATGP2613-100Recombinant human EF-1 alpha1/EEF1A1 protein
ATGP1022-100Recombinant human AIMP3/EEF1E1 protein
ATGP1022-020Recombinant human AIMP3/EEF1E1 protein
ATGP1022-500Recombinant human AIMP3/EEF1E1 protein
ATGP0946-100Recombinant human EF-1 beta/EEF1B2 protein
ATGP0946-020Recombinant human EF-1 beta/EEF1B2 protein
ATGP0946-250Recombinant human EF-1 beta/EEF1B2 protein
ATGP0946-010Recombinant human EF-1 beta/EEF1B2 protein

Alternative Products

CatalogName
35679-05111Human EEF1A1 Antibody
35679-05121Human EEF1A1 Antibody (Biotin Conjugate)
MBS2402692-01EEF1A1 Rabbit anti-Human Polyclonal Antibody
35679-05141Human EEF1A1 AssayLite™ Antibody (FITC Conjugate)
35679-05171Human EEF1A1 AssayLite™ Antibody (PerCP Conjugate)
35679-05151Human EEF1A1 AssayLite™ Antibody (RPE Conjugate)
35679-05161Human EEF1A1 AssayLite™ Antibody (APC Conjugate)
35679-05181Human EEF1A1 AssayLite Antibody (FITC, RPE, APC, PerCP Conjugate)
MBS2103247-01Human Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) Antibody Pair Kit (with Standard)
PT-A01505-01EEF1A1 Human

Popular Products