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Recombinant human EF-1 alpha1/EEF1A1 protein

CAT: 0112-ATGP2613-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2613-100Size:100 µg
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Description
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. Recombinant human EEF1A1 protein is expressed in E. coli.
Product Name Alternative
Eukaryotic translation elongation factor 1 alpha 1, Leukocyte receptor cluster (LRC) member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, EEF1A, EF1A, LENG7, EE1A1, EF1A1, EF1alpha1
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
50.1 kDa (462aa)
Additionnal Information
EEF1A1, Eukaryotic translation elongation factor 1 alpha 1, CCS-3, CCS3, EEF-1, EEF1A, eEF1A-1, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, PTI1, ATGP2613-10 µg, ATGP2613-20 µg, ATGP2613-50 µg, ATGP2613-100 µg, ATGP2613-250 µg, ATGP2613-500 µg, ATGP2613-1 mg, ATGP2613-10, ATGP2613-20, ATGP2613-50, ATGP2613-100, ATGP2613-250, ATGP2613-500, ATGP2613-1
References & Citations
Meriin AB, Zaarur N, et al. (2012) . J Cell Sci. 125 (Pt 11) :2665-74. ; ; Itagaki K, Naito T, et al. (2012) . J Biol Chem. 287 (19) :16037-46. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001393.1
Uniprot Accession Number
P68104
Species
Human
AA Sequence
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK ITKSAQKAQK AK