Recombinant human AIMP3/EEF1E1 protein

CAT: 0112-ATGP1022-500Size: 500 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP1022-500Size:500 µg
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Description
EEF1E1, also known as, eukaryotic translation elongation factor 1 epsilon-1, shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, this protein binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. upon DNA damage, it translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation. Recombinant human EEIF1E1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Eukaryotic translation elongation factor 1 epsilon-1, AIMP3, P18, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Multisynthetase complex auxiliary component p18, Elongation factor p18
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
21.9 kDa (194aa) confirmed by MALDI-TOF
Additionnal Information
EEF1E1, Eukaryotic translation elongation factor 1 epsilon-1, AIMP3, P18, ATGP1022-10 µg, ATGP1022-20 µg, ATGP1022-50 µg, ATGP1022-100 µg, ATGP1022-250 µg, ATGP1022-500 µg, ATGP1022-1 mg, ATGP1022-10, ATGP1022-20, ATGP1022-50, ATGP1022-100, ATGP1022-250, ATGP1022-500, ATGP1022-1
References & Citations
Quevillon S. et al. (1996) FEBS Lett. 395: 63-67.; ; Norcum MT. et al. (1991) J Biol Chem. 266:15398-405
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Cell Cycle
NCBI Accession Number
NP_004271
Uniprot Accession Number
O43324
Species
Human
AA Sequence
MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH

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