Recombinant human AIMP3/EEF1E1 protein

• Description:

  EEF1E1, also known as, eukaryotic translation elongation factor 1 epsilon-1, shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, this protein binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. upon DNA damage, it translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation. Recombinant human EEIF1E1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

• Product Name Alternative:

  Eukaryotic translation elongation factor 1 epsilon-1, AIMP3, P18, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Multisynthetase complex auxiliary component p18, Elongation factor p18

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Purity:

  > 95% by SDS-PAGE

• Molecular Weight:

  21.9 kDa (194aa) confirmed by MALDI-TOF

• Additionnal Information:

  EEF1E1, Eukaryotic translation elongation factor 1 epsilon-1, AIMP3, P18, ATGP1022-10 µg, ATGP1022-20 µg, ATGP1022-50 µg, ATGP1022-100 µg, ATGP1022-250 µg, ATGP1022-500 µg, ATGP1022-1 mg, ATGP1022-10, ATGP1022-20, ATGP1022-50, ATGP1022-100, ATGP1022-250, ATGP1022-500, ATGP1022-1

• References & Citations:

  Quevillon S. et al. (1996) FEBS Lett. 395: 63-67.; ; Norcum MT. et al. (1991) J Biol Chem. 266:15398-405

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

• Scientific Category:

  Cell Cycle

• NCBI Accession Number:

  NP_004271

• Uniprot Accession Number:

  O43324

• Species:

  Human

• AA Sequence:

  MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH