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Human BMP-8b antibody

CAT: 0112-ATGA0332-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0332-100Size:100 µL
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Description
Bone morphogenic proteins (BMPs) are members of the TGF beta superfamily. BMPs are involved in the induction of cartilage and bone formation. Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. BMP-8 is thought to be involved in early development, as detectable expression has not been found in adult organs. Two BMP-8 proteins exist, namely BMP-8A and BMP-8B (also designated OP-2), and are encoded by two distinct genes.
Product Name Alternative
Bone morphogenetic protein 8B, BMP8, OP2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BMP8b (264-402aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT13E6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
BMP8b, ATGA0332-10 µg, ATGA0332-20 µg, ATGA0332-50 µg, ATGA0332-100 µg, ATGA0332-250 µg, ATGA0332-500 µg, ATGA0332-1 mg, ATGA0332-10, ATGA0332-20, ATGA0332-50, ATGA0332-100, ATGA0332-250, ATGA0332-500, ATGA0332-1
References & Citations
Ozkaynak E., et al. (1992) J Biol Chem. 267 (35) :25220-25227.; ; Gregory SG., et al. (2006) Nature. 441 (7091) :315-321.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology
NCBI Accession Number
NP_001711
Uniprot Accession Number
P34820
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: BMP8b transfected 293T cell lysate The Recombinant BMP8b proteins (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0332-WB2.jpg}
IF Description
ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with ATGA0332 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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