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Human BMP-2 antibody

CAT: 0112-ATGA0281-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0281-050Size:50 µL
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Description
BMP-2 (Bone morphogenetic protein 2) is multi-functional growth factors that belong to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility.
Product Name Alternative
Bone morphogenetic protein 2, BMP-2A
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BMP2 (283-396aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT15B3
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CD159 antigen-like family member A, Bone Morphogenetic protein-2, Bone morphogenetic Protein 2, BMP-2A, BMP2A, BMP2 (monomer), BMP2, ATGA0281-10 µg, ATGA0281-20 µg, ATGA0281-50 µg, ATGA0281-100 µg, ATGA0281-250 µg, ATGA0281-500 µg, ATGA0281-1 mg, ATGA0281-010, ATGA0281-020, ATGA0281-050, ATGA0281-100, ATGA0281-250, ATGA0281-500, ATGA0281-01M
References & Citations
Chen D., et al. (2004) . Growth factors. 22 (4) :233-41. ; ; Schliephake H., et al. (2005) . Clin Oral Implants Res. 17 (6) :666-72.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001191
Uniprot Accession Number
P12643
WB Description
The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate
IF Description
ICC/IF analysis of BMP2 in Hep3B cells. The cell was stained with ATGA0281 (1:100) . The secondary antibody (green) was used Alexa Fluor 488.

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