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Human CRADD antibody

CAT: 0112-ATGA0327-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0327-100Size:100 µL
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Description
CRADD, also referred as RAIDD, is an adaptor protein that could interact with both caspase 2 and RIP that can promote apoptosis once activated. RAIDD (RIP-associated ICH-1/Ced-3 homologous protein with a death domain) has been identified as a RIP binding protein that also associates with members of the caspase family, providing a link between activation of the TNF-Rs and the triggering of the cysteine protease cascade. The amino-terminal domain of RAIDD shares significant homology with the prodomain of ICH-1 and mediates the binding of RAIDD to this cysteine protease.
Product Name Alternative
Caspase and RIP adapter with death domain, RAIDD, CRADD, Caspase and RIP adapter with death domain, Caspase and RIP adapter with death domain CASP2 and RIPK1 domain containing adaptor with death domain, Death adaptor molecule RAIDD, Death domain containing protein CRADD, MGC9163, RIP associated ICH1/CED3 homologous protein with death domain, RIP associated protein with a death domain.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CRADD (1-199aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1a κ
Clone
AT14G8
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CRADD, ATGA0327-10 µg, ATGA0327-20 µg, ATGA0327-50 µg, ATGA0327-100 µg, ATGA0327-250 µg, ATGA0327-500 µg, ATGA0327-1 mg, ATGA0327-10, ATGA0327-20, ATGA0327-50, ATGA0327-100, ATGA0327-250, ATGA0327-500, ATGA0327-1
References & Citations
Lin. Q., et al. (2012) J Immunol 188 (6) : 2493-2497.; ; Shen. Q., et al. (2014) Am J Transl Res 6 (5) : 538-547.; ; Ahmad. M., et al. (1997) Cancer Res 57 (4) : 615-619.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Apoptosis
NCBI Accession Number
NP_003796
Uniprot Accession Number
P78560
WB Description
The cell lysates of A549, HeLa, MCF-7 and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A549 cell lysate ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: MCF-7 cell lysate ; ; Lane 4.: 293T cell lysateThe Recombinant Human CRADD (50,100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Protein 50 ng ; ; Lane 2.: Recombinant Protein 100 ng{ATGA0327-WB2.jpg}
IF Description
ICC/IF analysis of CRADD in HeLa cells. The cell was stained with ATGA0327 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of CRADD in PC3 cells. The cell was stained with ATGA0327 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0327-IF2.jpg}

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