Human Bub3 antibody

CAT: 0112-ATGA0319-050Size: 50 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0319-050Size:50 µL
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Description
BUB3 (budding uninhibited by benzimidazoles 3 homolog) is a conserved component of the mitotic spindle assembly complex (MCC) . It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments.
Product Name Alternative
Mitotic checkpoint protein BuB3, BuB3L, hBuB3
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BUB3 (1-328aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT2H6
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Bub3, Mitotic checkpoint protein BuB3, BuB3L, hBuB3, ATGA0319-10 µg, ATGA0319-20 µg, ATGA0319-50 µg, ATGA0319-100 µg, ATGA0319-250 µg, ATGA0319-500 µg, ATGA0319-1 mg, ATGA0319-10, ATGA0319-20, ATGA0319-50, ATGA0319-100, ATGA0319-250, ATGA0319-500, ATGA0319-1
References & Citations
Loqarinho. E., et al. (2008) Mol Biol Cell 19 (4) : 1798-1813; ; Warren. C.D., et al. (2002) Mol Biol Cell 13 (9) : 3029-3041; ; Taylor. S.S., et al. (1998) J Cell Biol 142 (1) : 1-11
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000
Scientific Category
Cell Cycle
NCBI Accession Number
NP_004716
Uniprot Accession Number
O43684
WB Description
The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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