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Human CNBP antibody

CAT: 0112-ATGA0262-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0262-050Size:50 µL
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Description
CNBP is a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. It functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2.
Product Name Alternative
Cellular nucleic acid-binding protein, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CNBP (1-170aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT38F10
Conjugation
Unconjugated
Applications
ELISA, WB, FACS, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CNBP, Cellular nucleic acid-binding protein, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9, ATGA0262-10 µg, ATGA0262-20 µg, ATGA0262-50 µg, ATGA0262-100 µg, ATGA0262-250 µg, ATGA0262-500 µg, ATGA0262-1 mg, ATGA0262-10, ATGA0262-20, ATGA0262-50, ATGA0262-100, ATGA0262-250, ATGA0262-500, ATGA0262-1
References & Citations
McGrath CF, Buckman JS, et al. (2003) . J Virol. 77 (15) :8524-31.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:1000. Recommended starting dilution is 1:1000.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001120668
Uniprot Accession Number
P62633
WB Description
The cell lysate of 293T (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CNBP antibody (1:250 - 1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
FACS Description
Flow cytometry analysis of CNBP in MCF7 cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of CNBP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CNBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0262-IF1b.jpg} ICC/IF analysis of CNBP in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CNBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0262-IF2a.jpg} {ATGA0262-IF2b.jpg}

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