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Human ACOT7 antibody

CAT: 0112-ATGA0245-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0245-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ACOT7 is a member of the acyl coenzyme family. This protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this protein may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized.
Product Name Alternative
Acyl-CoA thioesterase 7, ACH1, ACT, BACH, CTE-II, hBACH, LACH, LACH1, MGC1126, RP1-120G22
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human ACOT7 (1-370aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT1D5
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
ACOT7, ATGA0245-10 µg, ATGA0245-20 µg, ATGA0245-50 µg, ATGA0245-100 µg, ATGA0245-250 µg, ATGA0245-500 µg, ATGA0245-1 mg, ATGA0245-10, ATGA0245-20, ATGA0245-50, ATGA0245-100, ATGA0245-250, ATGA0245-500, ATGA0245-1
References & Citations
Hunt MC., et al. (2005) J Lipid Res. 46, 2029-32.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_009205
Uniprot Accession Number
O00154
WB Description
The 293T cell lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT7 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
FACS Description
Flow cytometry analysis of ACOT7 in 293T cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of ACOT7 in U87MG cells. The cell was stained with ATGA0245 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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