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Human CISD1 antibody

CAT: 0112-ATGA0242-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0242-100Size:100 µL
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Description
CISD1 (CDGSH iron-sulfur domain-containing protein 1) is 108 amino acid protein. CISD1 plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions. This protein expression is reduced in cells derived from cystic fibrosis patients.
Product Name Alternative
DGSH iron-sulfur domain-containing protein 1, Cysteine transaminase CISD1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CISD1 (32-108aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1A8
Conjugation
Unconjugated
Applications
ELISA, WB, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CISD1, ATGA0242-10 µg, ATGA0242-20 µg, ATGA0242-50 µg, ATGA0242-100 µg, ATGA0242-250 µg, ATGA0242-500 µg, ATGA0242-1 mg, ATGA0242-10, ATGA0242-20, ATGA0242-50, ATGA0242-100, ATGA0242-250, ATGA0242-500, ATGA0242-1
References & Citations
Wiley, S.E., et al. (2007) J Biol Chem 282: 23745-23749.; ; Paddock, M.L., et al. (2007) Proc Natl Acad Sci USA 104: 14342-14347.; ; Taminelli, G.L., et al. (2008) Biochem Biophys Res Commun 365: 856-862.
Other References
He QQ, et al. MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection. (Sci Rep. 2016) {https://pubmed.ncbi.nlm.nih.gov/27748416/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_060934
Uniprot Accession Number
Q9NZ45
WB Description
The tissue lysate of Mouse liver (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: MCF7 cell lysate {ATGA0242-WB2.jpg}
FACS Description
Flow cytometry analysis of CISD1 in THP-1 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

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