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Human SAR1B antibody

CAT: 0112-ATGA0241-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0241-050Size:50 µL
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Description
SAR1B (GTP-binding protein SAR1b) is 198 amino acid protein. SAR1B is involved in transport from the endoplasmic reticulum to the Golgi apparatus. This protein is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Mutations in the SAR1B gene are associated with CMRD which is autosomal recessive disorder of severe fat malabsorption.
Product Name Alternative
GTP-binding protein SAR1b, ANDD, CMRD, GTBPB, SARA2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human SAR1B (1-198aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1C7
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
SAR1B, ATGA0241-10 µg, ATGA0241-20 µg, ATGA0241-50 µg, ATGA0241-100 µg, ATGA0241-250 µg, ATGA0241-500 µg, ATGA0241-1 mg, ATGA0241-10, ATGA0241-20, ATGA0241-50, ATGA0241-100, ATGA0241-250, ATGA0241-500, ATGA0241-1
References & Citations
Jones, B., et al. (2003) Nat Genet 34 (1) : 29-31.; ; Annesi, G., et al. (2007) Clin Genet 71 (3) : 288-9.; ; Treepongkaruna, S., et al. (2009) J Pediatr Gastroenterol Nutr 48 (3) : 370-3.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_057187
Uniprot Accession Number
Q9Y6B6
WB Description
The tissue lysate of Mouse muscle (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SAR1B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;
FACS Description
Flow cytometry analysis of SAR1B in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of SAR1B in Hep3B cells. The cell was stained with ATGA0241 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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