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Human MRRF antibody

CAT: 0112-ATGA0237-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0237-050Size:50 µL
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Description
Mitochondrial ribosome recycling factor, also known as MRRF, is a 262 amino acid protein that belongs to the ribosome recycling factor (RRF) family. Localized to mitochondria, MRRF is responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. It may increase the efficiency of translation by recycling ribosomes from one round of translation to another. MRRF belongs to the RRF family. 8 isoforms of the human protein are produced by alternative splicing.
Product Name Alternative
Ribosome-recycling factor mitochondrial isoform 1, Ribosome-recycling factor, mitochondrial isoform 1, MRFF, MTRRF, RRF
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human MRRF (56-262aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT7D10
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
MRRF, ATGA0237-10 µg, ATGA0237-20 µg, ATGA0237-50 µg, ATGA0237-100 µg, ATGA0237-250 µg, ATGA0237-500 µg, ATGA0237-1 mg, ATGA0237-10, ATGA0237-20, ATGA0237-50, ATGA0237-100, ATGA0237-250, ATGA0237-500, ATGA0237-1
References & Citations
Checcarelli, N. et al. (May 1994) . J Neurol Sci. 123 (1-2) :74-9.; ; Janosi, L. et al. (1996) . Biochimie. 78 (11-12) :959-69.; ; Zavialov, A.V. et al. (Jun 2005) . Mol Cell. 1018 (6) :675-86.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_620132
Uniprot Accession Number
Q96E11
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRRF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: HeLa cell lysate

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