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Human GIPC1/2 antibody

CAT: 0112-ATGA0223-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0223-050Size:50 µL
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Description
GIPC, GAIP interacting protein at the C terminus, is a PDZ domain containing protein that interacts with RGS-GAIP. GIPC was also identified as TIP-2, a protein that interacts with the viral oncoprotein TAX, which transactivates viral and cellular promoters through interactions with various transcription factors. GIPC specifically localizes to clusters of vesicles near the plasma membrane and participates in G protein-coupled signaling pathway involved in regulating clathrin-coated vesicular trafficking. GIPC also associates with membrane bound semaphorin F (M-SemF), which is involved in neuronal axon growth, and it appears to regulate the subcellular distribution of M-SemF in the brain.
Product Name Alternative
PDZ domain containing family member 2, SEMCAP2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human GIPC2 (1-315aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1G10
Conjugation
Unconjugated
Applications
ELISA, WB, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Enolase 2, ENO2, P09104, NP_001966.1, PDZ domain containing family member 2, PDZ domain containing family, member 2, SEMCAP2, GIPC, GIPC1, O14908, NP_974199, ATGA0223-10 µg, ATGA0223-20 µg, ATGA0223-50 µg, ATGA0223-100 µg, ATGA0223-250 µg, ATGA0223-500 µg, ATGA0223-1 mg, ATGA0223-010, ATGA0223-020, ATGA0223-050, ATGA0223-100, ATGA0223-250, ATGA0223-500, ATGA0223-01M
References & Citations
De Vries L., et al. (1998) . Proc Natl Acad Sci U S A 95 (21) : 12340-5.; ; Liu T F, Kandala G, Setaluri V (2001) . J. Biol. Chem. (United States) 276 (38) : 35768-77.; ; Hu, Liaoyuan A., et al. (2003) . J. Biol. Chem. (United States) 278 (28) : 26295-301.;
Other References
Wang Q, et al. Modulation of m-Opioid Receptor Signaling by RGS19 in SH-SY5Y Cells. (Mol Pharmacol. 2013) {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558815/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_060125.4
Uniprot Accession Number
Q8TF65
WB Description
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GIPC antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: GIPC1 recombinant protein; ; Lane 2.: GIPC2 recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GIPC antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: HeLa cell lysate {2004010182665280.jpg}
FACS Description
Flow cytometry analysis of GIPC in HeLa cells. The cell was stained with ATGA0223 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

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