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Human Myoglobin antibody

CAT: 0112-ATGA0217-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0217-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MB (Myoglobin) is an an iron- and oxygen-binding protein. It is found in abundance in the muscle tissue of vertebrates in general and in almost all mammals. MB (myglobin) is related to hemoglobin, which consists of four myoglobin-like subunits that form a tetramer and are responsible for carrying oxygen in blood. The only time myoglobin is found in the bloodstream is when it is released following muscle injury. It is an abnormal finding, and can be diagnostically relevant when found in blood.
Product Name Alternative
Myoglobin, PVALB
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human MB (1-154aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT6E10
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Myoglobin, ATGA0217-10 µg, ATGA0217-20 µg, ATGA0217-50 µg, ATGA0217-100 µg, ATGA0217-250 µg, ATGA0217-500 µg, ATGA0217-1 mg, ATGA0217-10, ATGA0217-20, ATGA0217-50, ATGA0217-100, ATGA0217-250, ATGA0217-500, ATGA0217-1
References & Citations
George A., et al. (2004) . Journal of Experimental Biology 207 (Pt 20) : 3441-6.; ; Akaboshi E (1985) . Gene 33 (3) : 241-9.; ; J. P. Collman., et al. (2004) . Chem. Rev. 104 (2) : 561-588.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cardiovascular
NCBI Accession Number
NP_976312
Uniprot Accession Number
P02144
WB Description
The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myoglobin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Muscle Tissue lysate; ; Lane 2.: Mouse Heart Tissue lysate

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