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Human NANS antibody

CAT: 0112-ATGA0210-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0210-050Size:50 µL
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Description
NANS (Sialic acids synthease) is an enzyme that functions in the biosynthetic pathways of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis.
Product Name Alternative
Sialic acid synthase, SAS, N acetylneuraminate synthase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human NANS (1-359aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1G6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
NANS, ATGA0210-10 µg, ATGA0210-20 µg, ATGA0210-50 µg, ATGA0210-100 µg, ATGA0210-250 µg, ATGA0210-500 µg, ATGA0210-1 mg, ATGA0210-10, ATGA0210-20, ATGA0210-50, ATGA0210-100, ATGA0210-250, ATGA0210-500, ATGA0210-1
References & Citations
Milstein O., et al. (2008) J Biol Chem. 283 (49) :34414-22.; ; Aldape MJ., et al. (2007) J Infect Dis. 195 (12) :1838-45.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_061819
Uniprot Accession Number
Q9NR45
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: HepG2 cell lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: NANS Transfected 293T cell lysate {ATGA0210-WB2.jpg}
IF Description
ICC/IF analysis of NANS in HeLa cells. The cell was stained with ATGA0210 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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