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Human CtBP1 antibody

CAT: 0112-ATGA0199-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0199-050Size:50 µL
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Description
The C-terminal-binding protein (CtBP) was originally identified as a cellular protein that interacts with a C-terminal motif of adenovirus E1A oncoproteins. CtBP is highly conserved in higher eukaryotes. Vertebrates contain two highly homologous CtBP proteins (CtBP1 and CtBP2) while invertebrates contain a single CtBP. CtBP family proteins share significant sequence homology with D-2-hydroxy acid dehydrogenases. Also, CtBP proteins associate with various proteins and functions as a co-repressor of transcription.
Product Name Alternative
C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CTBP1 (1-440aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT4D6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CtBP1, C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684., ATGA0199-10 µg, ATGA0199-20 µg, ATGA0199-50 µg, ATGA0199-100 µg, ATGA0199-250 µg, ATGA0199-500 µg, ATGA0199-1 mg, ATGA0199-10, ATGA0199-20, ATGA0199-50, ATGA0199-100, ATGA0199-250, ATGA0199-500, ATGA0199-1
References & Citations
Katsanis N, et al. (1998) . Genomics 47 (2) : 294-9.; ; Paliwal S, et al. (March 2006) . Mol. Cell. Biol. 26 (6) : 2360-72.; ; Chinnadurai G (2002) . Mol. Cell 9 (2) : 213-24.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Recommended starting dilution is 1:500
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001319
Uniprot Accession Number
Q13363
WB Description
The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: NIH3T3 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: AGS cell lysate ; ; Lane 5.: Raji cell lysate ; ; Lane 6.: A549 cell lysate ; ; Lane 7.: Jurkat cell lysate
IF Description
ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0199-IF1b.jpg} ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0199-IF2a.jpg} {ATGA0199-IF2b.jpg}

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