Products for Research Use Only
RegisterLogin

Human Bcl-2 antibody

CAT: 0112-ATGA0190-050Size: 50 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-ATGA0190-050Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Bcl-2 (B-cell lymphoma protein 2 alpha) is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apoptosis. Bcl-2 protein has anti-apoptotic functions through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) .
Product Name Alternative
BCL2 apoptosis regulator, B-cell CLL/lymphoma 2, PPP1R50, protein phosphatase 1, regulatory subunit 50
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Bcl-2 (1-211aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT1B5
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Bcl2, ATGA0190-10 µg, ATGA0190-20 µg, ATGA0190-50 µg, ATGA0190-100 µg, ATGA0190-250 µg, ATGA0190-500 µg, ATGA0190-1 mg, ATGA0190-10, ATGA0190-20, ATGA0190-50, ATGA0190-100, ATGA0190-250, ATGA0190-500, ATGA0190-1
References & Citations
Zamzami N., et al, . (1998) Oncogene. 16 (17) :2265-82.; ; Fernandez-Sarabia MJ., et al, . (1993) Nature. 366 (6452) :274-5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_000624
Uniprot Accession Number
P10415
WB Description
The cell lysate of NIH3T3 (35ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Bcl-2 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
FACS Description
Flow cytometry analysis of Bcl-2 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with monoclonal anti-human Bcl-2 antibody (1:500) with Texas Red (Red) .

Related Products

CatalogName
ATGP0297-020Recombinant human Bcl-2 protein
ATGP0297-100Recombinant human Bcl-2 protein
ATGP1542-100Recombinant human Bcl-2 related protein A1 protein
ATGP1542-020Recombinant human Bcl-2 related protein A1 protein
ATGP1542-004Recombinant human Bcl-2 related protein A1 protein
ATGP1457-250Recombinant human Bcl-10 protein
ATGP1457-050Recombinant human Bcl-10 protein
ATGP0454-020Recombinant human Bcl-w protein
ATGP1457-010Recombinant human Bcl-10 protein
ATGP0454-500Recombinant human Bcl-w protein

Alternative Products

CatalogName
ASA-B0933Human BCL-2 Antibody
MBS175568-01Anti-human BCL-2 antibody
GR126157Genorise® Human Bcl-2 Monoclonal Antibody
TMAB-07382Anti-Human Bcl-2 Antibody (6Z4)
32201-05121Human Bcl-2 Antibody (Biotin Conjugate)
GTR18188296-01Mouse Monoclonal human BCL-2 Antibody
GWB-BBP004Polyclonal Anti- human BCL-2 Antibody
BCL11-ARabbit anti-Human BCL-2 antibody
E-KAB-0497Human Bcl-2 Antibody Pair Set
32113-05111Human Bcl-2-like Protein 2 (BCL2L2) Antibody

Popular Products