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Recombinant human Bcl-2 protein

CAT: 0112-ATGP0297-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0297-020Size:20 µg
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Description
Bcl-2, also known as B-cell lymphoma protein 2 alpha, is an anti-apoptotic protein located primarily in the outer mitochondrial membrane that blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apotosis. Bcl-2 exerts its anti-apoptotic function through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . The Bcl-2 gene has been related with a number of cancers, including melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity. Recombinant Bcl-2 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Name Alternative
BCL2 apoptosis regulator, B-cell CLL/lymphoma 2, PPP1R50, protein phosphatase 1, regulatory subunit 50
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.4 kDa (231aa) confirmed by MALDI-TOF
Additionnal Information
Bcl2, B-cell lymphoma protein 2 alpha, B-cell lymphoma protein 2 alpha, B-cell lymphoma protein 2 alpha Apoptosis regulator Bcl 2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl-2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2., ATGP0297-10 µg, ATGP0297-20 µg, ATGP0297-50 µg, ATGP0297-100 µg, ATGP0297-250 µg, ATGP0297-500 µg, ATGP0297-1 mg, ATGP0297-10, ATGP0297-20, ATGP0297-50, ATGP0297-100, ATGP0297-250, ATGP0297-500, ATGP0297-1
References & Citations
Zamzami N., et al. (1998) Oncogene. 16 (17) :2265-82.; ; Fernandez-Sarabia MJ., et al. (1993) Nature. 366 (6452) :274-5.;
Other References
Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012) {https://pubmed.ncbi.nlm.nih.gov/22843993/}; ; Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27040940/}; ; Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017) {https://pubmed.ncbi.nlm.nih.gov/28536714/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 2mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_000624
Uniprot Accession Number
P10415
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D

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