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Human Aurora B antibody

CAT: 0112-ATGA0185-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0185-100Size:100 µL
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Description
The aurora family (A, B and C) are serine threonine kinases and key regulators of chromosome segregation during mitosis. Aurora kinase B is a chromosomal passenger protein that regulates chromosome segregation and cytokinesis. Aurora kinase B is associated with the level of genetic instability within tumours and patient survival. It is strongly expressed in exponentially proliferating bronchial epithelial cells in culture and that this expression is markedly reduced in confluent cells. It is also shown that almost all tumours show higher levels of Aurora kinase B expression than their matched normal lung tissues, which could therefore simply be a consequence of a higher proliferative index, or be typical of the progenitor cell and atypical of the bulk of normal lung cells.
Product Name Alternative
Serine/threonine-protein kinase 12, Aurora-B, AIK2, AIM1, ARK2, AurB, aurkb-sv1, aurkb-sv2, IPL1, STK12, aurkb, AIM1, AuRKB, Aurora 1, STK1, STK5, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora related kinase 2, Aurora/IPL1 related kinase 2, Serine/theronine kinase 12, Serine/threonine protein kinase 12
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Aurora kinase B (1-344aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT2B1
Conjugation
Unconjugated
Applications
ELISA, WB, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Aurora B, ATGA0185-10 µg, ATGA0185-20 µg, ATGA0185-50 µg, ATGA0185-100 µg, ATGA0185-250 µg, ATGA0185-500 µg, ATGA0185-1 mg, ATGA0185-10, ATGA0185-20, ATGA0185-50, ATGA0185-100, ATGA0185-250, ATGA0185-500, ATGA0185-1
References & Citations
Posch M, et al. (2010) J Cell Biol, 191 (1) :61-74.; ; Lisa L, et al. (2009) J Cell Biol, 186 (4) : 491-507.; ; S L Smith, et al. (2005) Br J Cancer, 93 (6) : 719-729.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cell Cycle
NCBI Accession Number
NP_004208
Uniprot Accession Number
Q96GD4
WB Description
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Aurora kinase B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human Aurora kinase A; ; Lane 2.: Recombinant Human Aurora kinase B
FACS Description
Flow cytometry analysis of Aurora B in LNCap cells. The cell was stained with ATGA0185 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

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