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Human G6PD antibody

CAT: 0112-ATGA0158-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0158-100Size:100 µL
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Description
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.
Product Name Alternative
Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6PDH
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human G6PD (35-506aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT2F6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
G6PD, ATGA0158-10 µg, ATGA0158-20 µg, ATGA0158-50 µg, ATGA0158-100 µg, ATGA0158-250 µg, ATGA0158-500 µg, ATGA0158-1 mg, ATGA0158-10, ATGA0158-20, ATGA0158-50, ATGA0158-100, ATGA0158-250, ATGA0158-500, ATGA0158-1, Glucose-6-phosphate 1-dehydrogenase, zwf, Glucose-6-phosphate 1-dehydrogenase G6PD, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p
References & Citations
Huang Y., et al, (2008) Mol Genet Metab. 93 (1) :44-53.; ; Zimny A., et al. (2003) Pol Arch Med Wewn. 110 (5) :1327-33.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_001035810.1
Uniprot Accession Number
P11413
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human G6PD antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Jurkat cell lysate;
IF Description
ICC/IF analysis of G6PD in HeLa cells. The cell was stained with ATGA0158 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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