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Human LITAF antibody

CAT: 0112-ATGA0152-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0152-100Size:100 µL
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Description
Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) mediates the expression of inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced processes. LITAF binds to STAT6B, a member of the STAT6 family forming a complex on the TNF-alpha promoter that modulates TNF activity. High levels of expression of LITAF mRNA have been observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen.
Product Name Alternative
Lipopolysaccharide-induced TNF factor, PIG7, SIMPLE, FLJ38636, TP53I7
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human LITAF (1-161aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT1F9
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
LITAF, ATGA0152-10 µg, ATGA0152-20 µg, ATGA0152-50 µg, ATGA0152-100 µg, ATGA0152-250 µg, ATGA0152-500 µg, ATGA0152-1 mg, ATGA0152-10, ATGA0152-20, ATGA0152-50, ATGA0152-100, ATGA0152-250, ATGA0152-500, ATGA0152-1
References & Citations
Atucchi A, et al., (2006) Inflamm Bowel Dis 13 (1) :120-121.; ; Tang X, et al., (2005) Proc Natl Acad Sci USA 102 (14) :5132-5137.; ; Tang X, et al., (2003) Proc Natl Acad Sci USA 100 (7) :4096-4101.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_004853
Uniprot Accession Number
Q99732
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LITAF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate;
IF Description
ICC/IF analysis of LITAF in LnCap cells. The cell was stained with ATGA0152 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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