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Human GAS7 antibody

CAT: 0112-ATGA0147-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0147-100Size:100 µL
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Description
Growth arrest-specific 7, also known as GAS7, is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development by promoting maturation and morphological differentiation of cerebellar neurons. Inhibition of GAS7 production in terminally differentiating cultures of embryonic murine cerebellum impedes neurite outgrowth. Conversely, the hyper-expression of GAS7 may play an important role in the initiation and development of human osteosarcoma.
Product Name Alternative
Growth arrest-specific 7 isoform b, KIAA0394, Growth arrest-specific 7, isoform b Growth arrest specific 7, GAS 7, GAS7, MGC1348, MLL/GAS7 fusion protein.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human GAS7 (1-416aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT4H8
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
GAS7, Growth arrest-specific 7 isoform b, KIAA0394, Growth arrest-specific 7, isoform b Growth arrest specific 7, GAS 7, GAS7, MGC1348, MLL/GAS7 fusion protein., ATGA0147-10 µg, ATGA0147-20 µg, ATGA0147-50 µg, ATGA0147-100 µg, ATGA0147-250 µg, ATGA0147-500 µg, ATGA0147-1 mg, ATGA0147-10, ATGA0147-20, ATGA0147-50, ATGA0147-100, ATGA0147-250, ATGA0147-500, ATGA0147-1
References & Citations
Chao CC, et al., (2005) J Neurosci Res. 81 (2) :153-62; ; She BR, et al., (2002) Exp Cell Res. 273 (1) :34-44
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.
Scientific Category
Neuroscience
NCBI Accession Number
NP_958836
Uniprot Accession Number
O60861
WB Description
The extracts of mouse brain (50 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAS7 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
IF Description
ICC/IF analysis of GAS7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of GAS7 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0147-IF2.jpg}

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