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Human STEAP1 antibody

CAT: 0112-AST0910-050Size: 50 µLDry Ice: NoHazardous: No
CAT#:0112-AST0910-050Size:50 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewings sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.
Product Name Alternative
Six transmembrane epithelial antigen of the prostate 1, six transmembrane epithelial antigen of the prostate, PRSS24
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human STEAP1 (1-70aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
J2D2
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, IHC
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
STEAP1, AST0910-10 µg, AST0910-20 µg, AST0910-50 µg, AST0910-100 µg, AST0910-250 µg, AST0910-500 µg, AST0910-1 mg, AST0910-10, AST0910-20, AST0910-50, AST0910-100, AST0910-250, AST0910-500, AST0910-1
References & Citations
David A. Rodeberg, et al. (2005) Clin Cancer Res. June 11 (12) : 4545-4552.; ; Pia M. Challita-Eid, et al., (2007) Cancer research June 67 (12) : 5798-5805.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_036581
Uniprot Accession Number
Q9UHE8
WB Description
The cell lysates (30ug) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: LNcap cell lysate; ; Lane 2.: Mouse Testis Tissue lysate;
IHC description
Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {AST0910-IHC1b.jpg}
IF Description
ICC/IF analysis of STEAP1 in LNCap cells. The cell was stained with AST0910 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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