Human STEAP1 antibody

• Description:

  Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewings sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.

• Product Name Alternative:

  Six transmembrane epithelial antigen of the prostate 1, six transmembrane epithelial antigen of the prostate, PRSS24

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human STEAP1 (1-70aa) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  J2D2

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, ICC/IF, IHC

• Purification Method:

  By protein-G affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  STEAP1, AST0910-10 µg, AST0910-20 µg, AST0910-50 µg, AST0910-100 µg, AST0910-250 µg, AST0910-500 µg, AST0910-1 mg, AST0910-10, AST0910-20, AST0910-50, AST0910-100, AST0910-250, AST0910-500, AST0910-1

• References & Citations:

  David A. Rodeberg, et al. (2005) Clin Cancer Res. June 11 (12) : 4545-4552.; ; Pia M. Challita-Eid, et al., (2007) Cancer research June 67 (12) : 5798-5805.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Cancer

• NCBI Accession Number:

  NP_036581

• Uniprot Accession Number:

  Q9UHE8

• WB Description:

  The cell lysates (30ug) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: LNcap cell lysate; ; Lane 2.: Mouse Testis Tissue lysate;

• IHC description:

  Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {AST0910-IHC1b.jpg}

• IF Description:

  ICC/IF analysis of STEAP1 in LNCap cells. The cell was stained with AST0910 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .