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Human NSD3 antibody

CAT: 0112-ANS0804-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ANS0804-100Size:100 µL
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Description
This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of histone H3 and is amplified in several tumor cell lines and primary breast carcinomas.
Product Name Alternative
Transcription elongation factor B polypeptide 3 binding protein 1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, MGC126766, MGC142029, NSD 3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, pp14328, Whistle, WHSC1 like protein 1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human NSD3 (383-660aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT7B4
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
NSD3, Transcription elongation factor B polypeptide 3 binding protein 1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, MGC126766, MGC142029, NSD 3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, pp14328, Whistle, WHSC1 like protein 1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1, ANS0804-10 µg, ANS0804-20 µg, ANS0804-50 µg, ANS0804-100 µg, ANS0804-250 µg, ANS0804-500 µg, ANS0804-1 mg, ANS0804-10, ANS0804-20, ANS0804-50, ANS0804-100, ANS0804-250, ANS0804-500, ANS0804-1
References & Citations
Angrand P.O, et al. (2001) Genomics 74 (1) :79-88; ; Stec I, et al. (2000) FEBS Lett 473 (1) :1-5
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_075447
Uniprot Accession Number
Q9BZ95
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: MCF7 cell lysate ; ; Lane 4.: NIH3T3 cell lysate ; ; Lane 5.: U87MG cell lysate

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