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Recombinant human MIF protein

CAT: 0112-ATGP3101-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3101-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MIF also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Macrophage migration inhibitory factor, GIF, GLIF, MMIF
Expression System
E.coli
Antigen Species
Human
Tag
GST-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
39.2 kDa (345aa) confirmed by MALDI-TOF
Additionnal Information
MIF, ATGP3101-10 µg, ATGP3101-20 µg, ATGP3101-50 µg, ATGP3101-100 µg, ATGP3101-250 µg, ATGP3101-500 µg, ATGP3101-1 mg, ATGP3101-10, ATGP3101-20, ATGP3101-50, ATGP3101-100, ATGP3101-250, ATGP3101-500, ATGP3101-1
References & Citations
Bernhagen J., et al (1994) Biochemistry. 33: 14144-55.; ; Bucala R., et al. (1996) FASEB J 10: 1607-13.
Other References
Garai J, et al. Tetralone derivatives are MIF tautomerase inhibitors and attenuate macrophage activation and amplify the hypothermic response in endotoxemic mice. (J Enzyme Inhib Med Chem. 2021) {https://pubmed.ncbi.nlm.nih.gov/34225560/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_002406
Uniprot Accession Number
P14174
Species
Human
AA Sequence
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

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