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Human IRF3 antibody

CAT: 0112-AIR0718-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-AIR0718-100Size:100 µL
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Description
Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection.
Product Name Alternative
IRF3, Interferon regulatory factor-3, IRF-3, Interferon regulatory factor 3, IRF 3
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human IRF3 (108-166aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
3F10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
IRF3, AIR0718-10 µg, AIR0718-20 µg, AIR0718-50 µg, AIR0718-100 µg, AIR0718-250 µg, AIR0718-500 µg, AIR0718-1 mg, AIR0718-10, AIR0718-20, AIR0718-50, AIR0718-100, AIR0718-250, AIR0718-500, AIR0718-1
References & Citations
DeFilippis VR et al., (2006) J Virol 80 (2) :1032-1037.; ; Pietila TE et al., (2007) J Immunol 178 (1) :253-261.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology, Hormones & Cytokines
NCBI Accession Number
NP_001562
Uniprot Accession Number
Q14653
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: Jurkat cell lysate
FACS Description
Flow cytometry analysis of IRF3 in MCF7 cells. The cell was stained with AIR0718 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) . Flow cytometry analysis of IRF3 in Jurkat cells. The cell was stained with AIR0718 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) . {2004010048549707.jpg}
IF Description
ICC/IF analysis of human HeLa cells stained with IRF3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.

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