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Human IRF5 antibody

CAT: 0112-ATGA0518-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0518-050Size:50 µL
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Description
Interferon regulatory factor 5 (IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs (IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.
Product Name Alternative
Interferon regulatory factor 5, irf5
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human IRF-5 (176-240aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
10T1
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
IRF5, ATGA0518-10 µg, ATGA0518-20 µg, ATGA0518-50 µg, ATGA0518-100 µg, ATGA0518-250 µg, ATGA0518-500 µg, ATGA0518-1 mg, ATGA0518-10, ATGA0518-20, ATGA0518-50, ATGA0518-100, ATGA0518-250, ATGA0518-500, ATGA0518-1
References & Citations
Mario B. et al. (2007) J. Virology 81 (9) :4473-4481.; ; Ning S. et al. (2005) J. Virology 79:11671-11676.; ; Mario B. et al. (2007) J. Virology 81 (9) :4473-4481.
Other References
Moen SH, et al. Human Toll-like Receptor 8 (TLR8) Is an Important Sensor of Pyogenic Bacteria, and Is Attenuated by Cell Surface TLR Signaling. (Front Immunol. 2019) {https://pubmed.ncbi.nlm.nih.gov/31214180/}; ; Guo J, et al. A promising role of interferon regulatory factor 5 as an early warning biomarker for the development of human non-small cell lung cancer. (Lung Cancer. 2019) {https://pubmed.ncbi.nlm.nih.gov/31447002/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology
NCBI Accession Number
NP_116032.1
Uniprot Accession Number
Q13568
WB Description
The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: THP-1 cell lysate
FACS Description
Flow cytometry analysis of IRF5 in THP-1 cells. The cell was stained with ATGA0518 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained with ATGA0518 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of IRF-5 in Raw264.7 cells. The cell was stained with ATGA0518 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010457189584.jpg}

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