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Human Geminin antibody

CAT: 0112-AGM0621-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-AGM0621-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Geminin is a 25 kDa protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions.
Product Name Alternative
GMNN, Geminin, Geminin, DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, OTTHuMP00000039393, RP3 369A17.3
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Geminin (1-209 aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
2H7
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Geminin, AGM0621-10 µg, AGM0621-20 µg, AGM0621-50 µg, AGM0621-100 µg, AGM0621-250 µg, AGM0621-500 µg, AGM0621-1 mg, AGM0621-10, AGM0621-20, AGM0621-50, AGM0621-100, AGM0621-250, AGM0621-500, AGM0621-1
References & Citations
McGarry TJ. et al., (1998) Cell. 93 (6) :1043-53.; ; Pitulescu M. et al., (2005) Cell Mol Life Sci. 62 (13) :1425-33.; ; Luo L. et al., (2004) Nature. 427 (6976) :749-53.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_056979
Uniprot Accession Number
O75496
WB Description
The cell lysate of K562 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Geminin (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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