Human CD44 antibody

• Description:

  CD44 is a broadly distributed transmembrane glycoprotein that plays a critical role in a variety of cellular behaviors, including adhesion, migration, invasion, and survival. CD44 mediates cell-cell and cell-matrix interactions in a large part through its affinity for hyaluronan (HA), a glycosaminoglycan constituent of extracellular matrices, but also potentially through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Variants of CD44 are expressed in tissues during development, including embryonic epithelia. known functions of CD44 are cellular adhesion (aggregation and migration), hyaluronate degradation, lymphocyte activation, lymph node homing, myelopoiesis and lymphopoiesis, angiogenesis, and release of cytokines. The functions of CD44 are principally dependant on cellular adhesion in one setting or another.

• Product Name Alternative:

  CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, LHR, MDU2, MDU3, MIC4

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human CD44 (21-145aa) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  5C10

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, ICC/IF, FACS

• Purification Method:

  By protein-G affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  CD44, ACD0826-10 µg, ACD0826-20 µg, ACD0826-50 µg, ACD0826-100 µg, ACD0826-250 µg, ACD0826-500 µg, ACD0826-1 mg, ACD0826-10, ACD0826-20, ACD0826-50, ACD0826-100, ACD0826-250, ACD0826-500, ACD0826-1

• References & Citations:

  Cichy, J. and Pure, E. (2003) J.Cell Biol. 161, 839-843.; ; Sneath RJ, Mangham DC (1998) Mol Pathol. 51 (4) :191-200.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot, ICC/IF and FACS nalysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Immunology

• NCBI Accession Number:

  NP_001001390

• Uniprot Accession Number:

  P16070

• WB Description:

  The recombinant protein (200ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD44 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human CD44 protein

• FACS Description:

  Flow cytometry analysis of CD44 in HeLa cells. The cell was stained with ACD0826 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) . Flow cytometry analysis of CD44 in PBMC cells. The cell was stained with ACD0826 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) . {ACD0826-FC2.jpg}

• IF Description:

  ICC/IF analysis of CD44 in HeLa cells. The cell was stained with ACD0826 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .