Human CD45 antibody

• Description:

  CD45 belongs to a family of heavily glycosylated, high molecular weight transmembrane glycoproteins expressed on all cells of haematopoietic origin. Structurally heterogeneous, CD45 consists of a family of eight isoforms ranging from 180, 000 to 220, 000 MW generated by alternative splicing of three exons differentially expressed on subpopulation of lymphocytes. CD45 protein tyrosine phosphatase activity plays a central role in T-cell activation. CD45 plays an important role in signal transduction inhibition or upregulation of various immunological functions

• Product Name Alternative:

  Protein tyrosine phosphatase, receptor type, C (PTPRC), CD45, B220, CD 45, CD45 antigen, GP180, L CA, LCA, Leukocyte common antigen, LY5, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase receptor type c polypeptide, PTPRC, SCID due to PTPRC deficiency, T200, T200 glycoprotein

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human CD45 (1029-1249aa) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  3G4

• Conjugation:

  Unconjugated

• Applications:

  ELISA, FACS

• Purification Method:

  By protein-G affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  CD45, protein tyrosine phosphatase, receptor type, C (PTPRC), CD45, B220, CD 45, CD45 antigen, GP180, L CA, LCA, Leukocyte common antigen, LY5, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase receptor type c polypeptide, PTPRC, SCID due to PTPRC deficiency, T200, T200 glycoprotein, ACD0802-10 µg, ACD0802-20 µg, ACD0802-50 µg, ACD0802-100 µg, ACD0802-250 µg, ACD0802-500 µg, ACD0802-1 mg, ACD0802-10, ACD0802-20, ACD0802-50, ACD0802-100, ACD0802-250, ACD0802-500, ACD0802-1

• References & Citations:

  Evgueni K , et al., (2005) Immunogenetics 57 (5) :374-383; ; Holmes N., (2006) Immunology 117 (2) :145-155; ; Francois S , et al., (2004) Immunology 113 (4) :441-452; ; Tara S , et al., (2003) Proc Natl Acad Sci U S A 100 (10) :5997-6002

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Immunology

• NCBI Accession Number:

  NM_002838

• Uniprot Accession Number:

  P08575

• FACS Description:

  Flow cytometry analysis of CD45 in Jurkat cells. The cell was stained with ACD0802 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .