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Human CEBP beta/CEBPB antibody

CAT: 0112-ABP0609-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ABP0609-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPalpha, C/EBPbeta, C/EBPdelta, C/EBPepsilon, and C/EBPgamma. Among these proteins, C/EBPbeta is usually highly expressed in liver, intestine, lung and adipose. C/EBPbeta has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP) . These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.
Product Name Alternative
CCAAT/enhancer-binding protein beta, Liver activator protein, LAP, liver-enriched transcriptional activator protein, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, NFIL6, Transcription factor 5, TCF-5, Interleukin 6-dependent DNA-binding protein, IL6DBP, CRP2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human C/EBP-beta (1-271 aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
47A1
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EBP-b, ABP0609-10 µg, ABP0609-20 µg, ABP0609-50 µg, ABP0609-100 µg, ABP0609-250 µg, ABP0609-500 µg, ABP0609-1 mg, ABP0609-10, ABP0609-20, ABP0609-50, ABP0609-100, ABP0609-250, ABP0609-500, ABP0609-1
References & Citations
Lekstrom-Himes J. et al. (1998) J Biol. Chem. 273, 28545-28548.; ; Parkin SE. et al. (2002) J Biol. Chem. 277:23563-23572.; ; Su WC. et al. (2003) J Biol. Chem. 278, 51150-51158.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005185
Uniprot Accession Number
P17676
WB Description
The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-beta antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate
IF Description
ICC/IF analysis of C/EBP-beta in HaLa cells. The cell was stained with ABP0609 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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