Human beta-Actin antibody

Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

Actin, cytoplasmic 1, Beta-actin

Human

Human

Recombinant human ACTB (1-375aa) purified from E. coli

AT4G6

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

0.2 mg/mL (determined by BCA assay)

B-Actin, beta actin, beta-actin, actin, ATGA0457, ATGA0570-10 µg, ATGA0570-20 µg, ATGA0570-50 µg, ATGA0570-100 µg, ATGA0570-250 µg, ATGA0570-500 µg, ATGA0570-1 mg, ATGA0570-10, ATGA0570-20, ATGA0570-50, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-1

Gunning PW., et al. (2015) Journal of Cell Science. 128 (11) : 2009-2019.; ; Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163 (4) : 673-8.

Yang YJ et al., Liquid Chromatography/Tandem Mass Spectrometry Analysis of Sophora flavescens Aiton and Protective Effects against Alcohol-Induced Liver Injury and Oxidative Stress in Mice. (Antioxidants (Basel) . 2024) {https://pubmed.ncbi.nlm.nih.gov/38790646/}; ; Yang YJ et al., Korean Mistletoe (Viscum album var. coloratum) Ethanol Extracts Enhance Intestinal Barrier Function and Alleviate Inflammation. (Antioxidants (Basel) 2025) {https://pubmed.ncbi.nlm.nih.gov/40227441}; ; Kim MJ et al., Potential Chondroprotective Effect of Artemisia annua L. Water Extract on SW1353 Cell. (Int. J. Mol. Sci. 2025) {https://pubmed.ncbi.nlm.nih.gov/40076528/}; ; Han JY et al., Cytoplasmic HMGB1 promotes the activation of JAK2-STAT3 signaling and PD-L1 expression in breast cancer. (Mol Med. 2025) {https://pubmed.ncbi.nlm.nih.gov/40389855/}; ; Kim MJ et al., Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition. (Sci Rep. 2025) {https://pubmed.ncbi.nlm.nih.gov/39827243/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_001092

P60709

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: PC3 cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: MCF7 cell lysate; ; Lane 6.: U87-MG cell lysate; ; Lane 7.: NIH-3T3 cell lysateThe mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Kidney tissue lysate; ; Lane 2.: Liver tissue lysate; ; Lane 3.: Spleen tissue lysate; ; Lane 4.: Bone marrow tissue lysate; ; Lane 5.: Stomach tissue lysate {ATGA0570-WB2.jpg}The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: ACTB antibody 1:1000; ; Lane 2.: ACTB antibody 1:3000; ; Lane 3.: ACTB antibody 1:5000; ; Lane 4.: ACTB antibody 1:10000 {ATGA0570-WB3.jpg}