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Human beta-Actin antibody

CAT: 0112-ATGA0606-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0606-050Size:50 µL
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Description
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Product Name Alternative
Actin, cytoplasmic 1, Beta-actin
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human ACTB (1-375aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT4G6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
0.2 mg/mL (determined by BCA assay)
Additionnal Information
B-Actin, beta actin, beta-actin, actin, ATGA0570-10 µg, ATGA0570-20 µg, ATGA0570-50 µg, ATGA0570-100 µg, ATGA0570-250 µg, ATGA0570-500 µg, ATGA0570-1 mg, ATGA0570-010, ATGA0570-020, ATGA0570-050, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-01M, ATGA0606-10 µg, ATGA0606-20 µg, ATGA0606-50 µg, ATGA0606-100 µg, ATGA0606-250 µg, ATGA0606-500 µg, ATGA0606-1 mg, ATGA0606-010, ATGA0606-020, ATGA0606-050, ATGA0606-100, ATGA0606-250, ATGA0606-500, ATGA0606-01M
References & Citations
Gunning PW., et al. (2015) Journal of Cell Science. 128 (11) : 2009-2019.; ; Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163 (4) : 673-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_001092
Uniprot Accession Number
P60709
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: PC3 cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: MCF7 cell lysate; ; Lane 6.: U87-MG cell lysate; ; Lane 7.: NIH-3T3 cell lysateThe mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Kidney tissue lysate; ; Lane 2.: Spleen tissue lysate; ; Lane 3.: Bone marrow tissue lysate; ; Lane 4.: Stomach tissue lysate; ; Lane 5.: Liver tissue lysate {2503310001584356.jpg}The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: ACTB antibody 1:1000; ; Lane 2.: ACTB antibody 1:3000; ; Lane 3.: ACTB antibody 1:5000; ; Lane 4.: ACTB antibody 1:10000 {2503310001814296.jpg}
IF Description
ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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