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Human BID antibody

CAT: 0112-ABI0923-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ABI0923-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into the outer mitochondrial membrane. Bax is believed to induce the opening of the mitochondrial voltage-dependent anion channel. This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria leading to activation of caspases.
Product Name Alternative
BH3 interacting domain death agonist isoform 2, FP497, MGC15319, MGC42355, BH3 interacting domain death agonist isoform 2, BID, BH3 interacting domain death agonist isoform 2 Apoptotic death agonist, Apoptotic death agonist BID, BH3 interacting domain death agonist, HGNC:1050, BH3 interacting domain death agonist p11, BH3 interacting domain death agonist p13, BH3 interacting domain death agonist p15, BID isoform ES (1b), BID isoform L (2), Desmocollin type 4, Human BID coding sequence, OTTHuMP00000196197, p11 BID, p13 BID, p15 BID, p22 BID
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BID (1-195aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
4D3
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
BID, ABI0923-10 µg, ABI0923-20 µg, ABI0923-50 µg, ABI0923-100 µg, ABI0923-250 µg, ABI0923-500 µg, ABI0923-1 mg, ABI0923-10, ABI0923-20, ABI0923-50, ABI0923-100, ABI0923-250, ABI0923-500, ABI0923-1
References & Citations
Zha J., et al. (2000) Science. 290:1761.; ; Luo X., et al. (1998) Cell. 94:481.; ; Li H., et al. (1998) Cell. 94:491.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Apoptosis
NCBI Accession Number
NP_001187
Uniprot Accession Number
P55957
WB Description
The Jurkat cell lysate (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BID antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BID antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: A549 cell lysate; ; {ABI0923-WB2.jpg}
IF Description
ICC/IF analysis of BID in HeLa cells. The cell was stained with ABI0923 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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