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Human AK3 antibody

CAT: 0112-AAK0604-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-AAK0604-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Adenylate kinase (Ak; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.
Product Name Alternative
Adenylate kinase, Ak6, FIX, Ak3L1, AkL3L, AkL3L1, adenylate kinase 3, adenylate kinase 6, adenylate kinase 3 like 1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Ak3 (adenylaste kinase isozyme 3) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
SJB3-36
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, IHC, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
AK3, AAK0604-10 µg, AAK0604-20 µg, AAK0604-50 µg, AAK0604-100 µg, AAK0604-250 µg, AAK0604-500 µg, AAK0604-1 mg, AAK0604-10, AAK0604-20, AAK0604-50, AAK0604-100, AAK0604-250, AAK0604-500, AAK0604-1
References & Citations
Noda, L.H. et al. (1973) The Enzymes. Vol. 8, 279-305.; ; Khoo, J.C. et al. (1972) Biochim. Biophys. Acta 268, 98-101.; ; Tomasselli, A.G. et al. (1979) Eur.J.Biochemolecules 93, 257-262.; ; Lee, Y. et al. (1998) J. Biochem. 123, 47-54.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_057366
Uniprot Accession Number
Q9UIJ7
WB Description
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Recombinant Human AK1; ; Lane 2. : Recombinant Human AK2; ; Lane 3. : Recombinant Human AK3 The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: Lncap cell lysate; ; {2004010017118777.jpg}
IHC description
Human liver tissue was incubated with anti-human Ak3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.
FACS Description
Flow cytometry analysis of AK3 in jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of AK3 in U87MG cells. The cell was stained with AAK0604 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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