MSH3 Polyclonal Antibody

• Background:

  Component of the post-replicative DNA mismatch repair system (MMR) . Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

• Synonyms:

  DUP; MRP1; DNA mismatch repair protein Msh3; hMSH3; Divergent upstream protein; Mismatch repair protein 1; MSH3; DUC1; DUG

• Gene ID:

  4437

• Swiss Prot:

  P20585

• Cellular Locus:

  Cytoplasm, Nucleus

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse, Rat

• Immunogen:

  521-620/1137

• Target:

  MSH3

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human MSH3

• Applications:

  WB, IHC-P, IHC-F, IF

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (WB=1:500-2000), IHC-P (IHC-P=1:100-500), IHC-F (IHC-F=1:100-500), IF (IF=1:100-500)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  4437

• Predicted Cross Reactivity:

  Cow, Pig, Horse, Rabbit