Phospho-MAP3K8 (Ser400) Polyclonal Antibody

• Background:

  Required for lipopolysaccharide (LPS) -induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

• Synonyms:

  COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT; Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; MAP3K8

• Gene ID:

  1326

• Swiss Prot:

  P41279

• Modification Site:

  Ser400

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse, Rat

• Target:

  Phospho-MAP3K8 (Ser400)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic phosphopeptide derived from human MAP3K8/Tpl2 around the phosphorylation site of Ser400

• Applications:

  WB, ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Phosphorylation

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Specificity:

  This phosphorylation site is homologous across the listed species.

• Gene ID URL:

  1326

• Predicted Cross Reactivity:

  Dog, Cow, Pig, Horse, Chicken, Rabbit