MAP3K8 Antibody (Phospho-Ser400)

• Gene Name:

  Mitogen-activated protein kinase kinase kinase 8

• Gene Aliases:

  COT, EST, ESTF, TPL2, AURA2, MEKK8, Tpl-2, c-COT

• Gene ID:

  1326; 26410; 116596

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from human Cot around the phosphorylation site of S400. Location: 340-420aa.

• Target:

  This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  IHC

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Reconstitution:

  Store at -20C. Avoid repeated freeze/thaw cycles.

• Molecular Weight:

  60 kDa

• Fragment:

  IgG

• Specificity:

  Phospho-Cot (S400) Polyclonal Antibody detects endogenous levels of Cot protein only when phosphorylated at S400.

• NCBI Gene Symbol:

  MAP3K8

• Host or Source:

  Rabbit

• Gene Name URL:

  MAP3K8

• CAS Number:

  9007-83-4