Phospho-eIF4E (Ser209) Polyclonal Antibody

• Background :

  Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

• Synonyms :

  CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

• Gene ID :

  1977

• Swiss Prot :

  P06730

• Modification Site :

  Ser209

• Cellular Locus :

  Cytoplasm, Nucleus

• Host :

  Rabbit

• Cross Reactivity :

  Human, Mouse, Rat, Zebrafish

• Target :

  Phospho-eIF4E (Ser209)

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 [SG (p-S) TT]

• Applications :

  WB, ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification :

  Purified by Protein A.

• Concentration :

  1µg/µl

• Dilution :

  WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Phosphorylation

• Storage Conditions :

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL :

  1977

• Predicted Cross Reactivity :

  Dog, Cow, Pig, Horse, Chicken, Rabbit