H2DCFDA (DCFH-DA)

CAT: 0957-S9687-04Size: 1 gDry Ice: NoHazardous: No

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CAT#:0957-S9687-04Size:1 g

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Description

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) production. It is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation. It is used in sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay. (Ex/Em=488/525 nm)

CAS Number

4091-99-0

Synonyms

2',7'-Dichlorodihydrofluorescein diacetate

Target

ROS

Activity

NF-κB

Molecular Formula

C24H16Cl2O7

Storage Conditions

3 years -20°C (in the dark) powder

Chemical Information

2',7'-Dichlorodihydrofluorescein diacetate

CAS Number	4091-99-0
PubChem CID	77718
IUPAC Name	2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
Molecular Formula	C24H16Cl2O7
Molecular Weight	487.3
XLogP	5.2
Topological Polar Surface Area	99.1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	6
Heavy Atom Count	33
Formal Charge	0
Complexity	717

SMILES

CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl

InChI

InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChIKey

PXEZTIWVRVSYOK-UHFFFAOYSA-N

Chemical Structure

2D Structure

CAS: 4091-99-0

CID: 77718

Formula: C24H16Cl2O7

MW: 487.3

Interactive 3D Structure

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View on PubChem Raw 3D data

Computed Properties

Property	Value
Molecular Weight	487.3
XLogP3	5.2
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	6
Exact Mass	486.0273082
Monoisotopic Mass	486.0273082
Topological Polar Surface Area	99.1 Å²
Heavy Atom Count	33
Formal Charge	0
Complexity	717
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	1
Compound Is Canonicalized	Yes

Alternative Products

Catalog	Name
C14414-01	H2DCFDA (DCFH-DA)
D6472-01	DCFH-DA (ROS Fluorescent Probe)
KP06003-1000	DCFH-DA probe (Intracellular ROS Assay)
KP06005-500	Multiprobe REDOX Assay Kit (DHE + DHR123 + DCFH-DA)
GTR19332396-01	H2DCFDA
R252-10	ROS Assay Kit -Highly Sensitive DCFH-DA-, 100 tests
15204-AAT	DCFH-DA [2',7'-Dichlorodihydrofluorescein diacetate] CAS 4091-99-0
R253-10	ROS Assay Kit -Photo-oxidation Resistant DCFH-DA-, 100 tests
HY-D1713	CM-H2DCFDA
RM-C180602-01	Carboxy-H2Dcfda

H2DCFDA (DCFH-DA)

Chemical Information

Alternative Products

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