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H2DCFDA

CAT: 0013-GTR19332396-01Size: 1 ml x 10 mM (in DMSO)Dry Ice: NoHazardous: No
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CAT#:0013-GTR19332396-01Size:1 ml x 10 mM (in DMSO)
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
H2DCFDA (DCFH-DA) is a cell-permeable fluorogenic probe widely used for detecting intracellular reactive oxygen species (ROS) in both in vitro and in vivo studies, with excitation/emission maxima at 488/525 nm. Its applications span research fields including oxidative stress, apoptosis, and inflammatory response monitoring.
CAS Number
4091-99-0
Product Name Alternative
Reactive Oxygen Species, ReactiveOxygenSpecies, DCFH-DA, H2DCFDA, H-2DCFDA, Inhibitor, inhibit, 2',7'-Dichlorodihydrofluorescein, 2',7'-Dichlorodihydrofluorescein Diacetate, 2',7'-Dichlorodihydrofluorescein diacetate
Field of Research
Immunology & Inflammation, Metabolism Research, Signal Transduction
Purity
97.04% (May vary between batches)
Solubility
Ethanol:14.29 mg/mL (29.33 mM) ; DMSO:257.5 mg/mL (528.43 mM) ;10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (10.26 mM)
Molecular Weight
487.29
Storage Conditions
-20°C
Notes
For research use only.

Chemical Information

2',7'-Dichlorodihydrofluorescein diacetate
CAS Number4091-99-0
PubChem CID77718
IUPAC Name2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
Molecular FormulaC24H16Cl2O7
Molecular Weight487.3
XLogP5.2
Topological Polar Surface Area99.1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count7
Rotatable Bond Count6
Heavy Atom Count33
Formal Charge0
Complexity717
SMILES
CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
InChI
InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChIKey
PXEZTIWVRVSYOK-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of 2',7'-Dichlorodihydrofluorescein diacetate
CAS: 4091-99-0
CID: 77718
Formula: C24H16Cl2O7
MW: 487.3
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight487.3
XLogP35.2
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count7
Rotatable Bond Count6
Exact Mass486.0273082
Monoisotopic Mass486.0273082
Topological Polar Surface Area99.1 Ų
Heavy Atom Count33
Formal Charge0
Complexity717
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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MBS8579549-01ROS Probes H2DCFDA
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