BUB3 Recombinant Antibody

• Background:

  Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

• Synonyms:

  Mitotic checkpoint protein BUB3; BUB3

• Gene ID:

  9184

• Swiss Prot:

  O43684

• Cellular Locus:

  Nucleus

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse, Rat

• Target:

  Bub3

• Clonality:

  Recombinant

• Conjugation:

  Unconjugated

• Source:

  Recombinant protein corresponding to the N-terminus of human Bub3.

• Applications:

  WB, FCM, IHC-P, IF (IHC-P), IF (ICC), IP

• Purification:

  Purified by Protein A.

• Concentration:

  1ug/ul

• Dilution:

  WB (1:300-5000), FCM (1:20-100), IHC-P (1:200-400), IF (IHC-P) (1:50-200), IF (ICC) (1:50-200), IP (1-2ug)

• Buffer:

  0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  9184

• Isotype:

  IgG