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Bub3 Polyclonal Antibody, RBITC Conjugated

CAT: 0498-bs-7835R-RBITCSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-7835R-RBITCSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Synonyms
BUB3L; hBUB3; Mitotic checkpoint protein BUB3; BUB3
Gene ID
9184
Swiss Prot
O43684
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Rat
Immunogen
201-300/328
Target
Bub3
Clonality
Polyclonal
Isotype
IgG
Conjugation
RBITC
Source
KLH conjugated synthetic peptide derived from human Bub3
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
570nm/595nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
9184
Predicted Cross Reactivity
Human, Mouse, Dog

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