Products for Research Use Only

Phospho-Src (Tyr529) Antibody

CAT: 0800-FY12031Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-FY12031Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
Western blot: 1:500-1:2000, Immunocytochemistry/Immunofluorescence: 1:50-1:200
UniProt
P12931
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human Phospho-Src (Y529) was used as the immunogen for the Phospho-Src (Tyr529) antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
32S01
Applications
WB, ICC, IF
Purity
Affinity-chromatography
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.
Limitations
This Phospho-Src (Tyr529) antibody is available for research use only.
Storage Conditions
Store the Phospho-Src (Tyr529) antibody at -20°C.
Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the Phospho-Src (Tyr529) antibody should be determined by the researcher.
Image Legend
All lanes use the antibody at 1:3K dilution for 1 hour at room temperature. Expected size for c-Src (phospho-Tyr529) ~60 kDa. Observed (possible) doublet at ~55 kDa and ~60 kDa likely reflects full-length and slightly modified/truncated phosphorylated forms of c-Src, consistent with literature indicating Src family kinases migrate in the 55-62 kDa range.

UniProtKB · P12931

Proto-oncogene tyrosine-protein kinase Src

SRC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P12931
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SRC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.10.2
Processing
—
Secondary accessions
E1P5V4, Q76P87, Q86VB9, Q9H5A8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Biological process

Cell adhesionCell cycleHost-virus interactionImmunity

Cellular component

Cell junctionCell membraneCytoplasmCytoskeletonMembraneMitochondrionMitochondrion inner membraneNucleus

Disease

Disease variantProto-oncogene

Molecular function

KinaseTransferaseTyrosine-protein kinase

PTM

LipoproteinMyristatePhosphoproteinUbl conjugation

Domain

SH2 domainSH3 domain