Phospho-Src Antibody (pY215)

CAT: 0800-F48452-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
CAT#:0800-F48452-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P12931
Host
Rabbit
Reactivity
Human
Immunogen
This phospho-Src antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY215 of human Src.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-Src antibody is available for research use only.
Storage Conditions
Aliquot the phospho-Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Chicken, Mouse, Rat, Xenopus, Zebrafish
Image Legend
FG pancreatic carcinoma cell lines stably expressing vector alone (FG-V), the b3 integrin subunit (FG-b3), or a b3 truncation mutant (FG-759x) probed with phospho-Src antibody. Ab was diluted 1:500.
Product Datasheet
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