PCGF6 Antibody / Polycomb group RING finger protein 6

• Specifications:

  Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  Q9BYE7

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human PCGF6 was used as the immunogen for the PCGF6 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS

• Purity:

  Immunogen affinity purified

• Format:

  Lyophilized

• Buffer:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Limitations:

  This PCGF6 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the PCGF6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

• Applications Notes:

  Optimal dilution of the PCGF6 antibody should be determined by the researcher.

• Image Legend:

  Flow Cytometry analysis of U251 cells using anti-PCGF6 antibody. Overlay histogram showing U251 cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCGF6 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.