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EIF4A3 Antibody / Eukaryotic initiation factor 4A-III

CAT: 0800-FY13354Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-FY13354Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P38919
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human EIF4A3 recombinant protein (Position: R14-I411) was used as the immunogen for the EIF4A3 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, ICC/IF, IP, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This EIF4A3 antibody is available for research use only.
Storage Conditions
After reconstitution, the EIF4A3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the EIF4A3 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Immunofluorescent staining of EIF4A3 using anti-EIF4A3 antibody (green) and anti-Beta Tubulin antibody (red) . EIF4A3 was detected in an immunocytochemical section of human A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-EIF4A3 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

UniProtKB · P38919

Eukaryotic initiation factor 4A-III

IF4A3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P38919
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EIF4A3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
eIF-4A-III, eIF4A-III
EC number
3.6.4.13
Processing
—
Secondary accessions
Q15033, Q6IBQ2, Q96A18
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Ligand

ATP-bindingNucleotide-binding

Cellular component

CytoplasmNucleusSpliceosome

Disease

Disease variant

Molecular function

HelicaseHydrolaseRNA-binding

Biological process

mRNA processingmRNA splicingmRNA transportNonsense-mediated mRNA decayrRNA processingTranslation regulationTransport

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