Eukaryotic Translation Initiation Factor 6 / EIF6

• Description:

  EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

• Specifications:

  Western blot: 0.1-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, ICC (FFPE) : 0.5-1 µg/mL

• UniProt:

  P56537

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids 66-210 of the human protein were used as the immunogen for the Eukaryotic Translation Initiation Factor 6 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, ICC

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Eukaryotic Translation Initiation Factor 6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Eukaryotic Translation Initiation Factor 6 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Eukaryotic Translation Initiation Factor 6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Eukaryotic Translation Initiation Factor 6 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear and cytoplasmic

• Image Legend:

  Western blot testing of 1) rat heart, 2) rat liver, 3) mouse liver, 4) human placenta, 5) COLO320 and 6) HeLa lysate with Eukaryotic Translation Initiation Factor 6 antibody. Expected molecular weight ~27 kDa.